Insertion junction: CLIP2.001539_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.001539
Chromosome:plastome
Location:32928
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802283 rps4,ChreCp020,2716955 30S ribosomal protein S4; (1 of 1) PTHR11831:SF4 - 37S RIBOSOMAL PROTEIN NAM9, MITOCHONDRIAL CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCCATGACTAATAAGTTGGCGTGCTGCTGGAATTGTAGGTGCCATATT
Internal bar code:TGTAAGGGGGTTTCGTCAGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:145
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCAAGGAAAATGTTGCGG
Suggested primer 2:CGCCACTGACGTCCACTAAT