Insertion junction: CLIP2.001540_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.001540
Chromosome:chromosome 7
Location:5813833
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g353500 CGLD5A ethylene response element DNA-binding domain containing protein; (1 of 1) PTHR31190:SF30 - ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 15-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCCCACTCCCCGTATGACCCCCTGCCCGACAGGAACACCCCACTGTTC
Internal bar code:TGAATGGTCAGTTACTCCGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3443
LEAP-Seq percent confirming:98.5075
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:67

Suggested primers for confirmation by PCR

Suggested primer 1:GCGACTGCGTAATTCGTGTC
Suggested primer 2:CTGCTGTTGCTGTTGTTGCT