Insertion junction: CLIP2.001542_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.001542
Chromosome:chromosome 7
Location:1677393
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325550 KDG3 Diacylglycerol kinase; (1 of 2) K00901 - diacylglycerol kinase (ATP) (dgkA, DGK) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAACCCGTGCTTCTTCTCGTCTGCCTCGCTGGTGTTAGGGCCCCACAGG
Internal bar code:ATAGTACTTTGGGTAAGTGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2123
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCGGTCAAGATTCCCAAG
Suggested primer 2:GCACTTTAACGTCCCATCGC