| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.001552 |
| Chromosome: | chromosome 14 |
| Location: | 1338935 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616700 | (1 of 2) 2.6.1.57 - Aromatic-amino-acid transaminase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGTAGGTGTAGCCTTGTTCGACCTCATTGTATCCATAAGTGTTGACA |
| Internal bar code: | CATCACTTTGAGGGCAGAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1142 |
| LEAP-Seq percent confirming: | 7.5 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGGGTGGCTGAAACATG |
| Suggested primer 2: | ATGGCATGCGCGTTTACATC |