| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.001573 |
| Chromosome: | chromosome 12 |
| Location: | 7225020 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559000 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCACACGGAAAGTCACTCACCGGAAGCCGCAGACTCCGCGCGGCCTGA |
| Internal bar code: | TGAGGATTTAGGCGGCGGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3319 |
| LEAP-Seq percent confirming: | 99.1736 |
| LEAP-Seq n confirming: | 120 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 121 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGCTGGTGAGTTGGTTGG |
| Suggested primer 2: | ATGGAAACGAGGGCGGATTT |