Insertion junction: CLIP2.001603_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.001603
Chromosome:chromosome 3
Location:313155
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g144504 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGTGGAAGACCGCAAGACCAACATCGACTTTTGAATGCAAAAGTGGG
Internal bar code:GAAAGGGCCGGGGTAGGCAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2562
LEAP-Seq percent confirming:95.7447
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAGCACTCAACCACACG
Suggested primer 2:CGCAGGTAGGCCGATATTGT