Insertion junction: CLIP2.001610_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.001610
Chromosome:chromosome 1
Location:3297010
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g021200 CGL47 conserved protein with F-box domain; (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTGGCATGGTTGACTCCGAATGGCCAACACCACTCGCGAGCTGCAGCA
Internal bar code:CACAAGTCTGACGTCCTAGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1454
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGGTATGCACGCTAGTA
Suggested primer 2:CACGGATGTACATAGCCCCC