Insertion junction: CLIP2.001618_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.001618
Chromosome:chromosome 3
Location:6065435
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g190150 CGL1 (1 of 2) PF11833 - Protein of unknown function (DUF3353) (DUF3353); Conserved in the Green Lineage 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTACAGCACTTCTCAAGCACGTGCCGAGCATGGAGGGGCAACTCTCGA
Internal bar code:CCGACGAGTCCGAGTTGCTAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1594
LEAP-Seq percent confirming:63.4146
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGTGGTGATGATGTGCAT
Suggested primer 2:GTCGTCTACCCCTCTGGAGT