| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.001629 |
| Chromosome: | chromosome 6 |
| Location: | 8411404 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g308100 | ECH2 | Enoyl-CoA hydratase 2; (1 of 2) 4.2.1.119 - Enoyl-CoA hydratase 2 / ECH2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGGGGCAAGGAGGGGTTTAATAATGGAACAAGCCCGATAAGGAGGTG |
| Internal bar code: | TTTTTGCAAAATTAGTCGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 525 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAAGCGTGCCGTGTAAA |
| Suggested primer 2: | TGTGATGCTGTTGCTGCAAC |