| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.001631 |
| Chromosome: | chromosome 5 |
| Location: | 378345 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTGTCCTTTCTTTCGGTCCTCCCCGACTAGTGCGCCGGGACCGTCCC |
| Internal bar code: | TATTATGCGATGAATAGTAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5855 |
| LEAP-Seq percent confirming: | 65.3846 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCAATGCTACCCACGAA |
| Suggested primer 2: | CCATTCAAGGTGCGCTATGC |