| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.001660 |
| Chromosome: | chromosome 1 |
| Location: | 7398206 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052100 | PRPL18 | (1 of 1) K02881 - large subunit ribosomal protein L18 (RP-L18, MRPL18, rplR); Chloroplast ribosomal protein L18 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGTGATGATGCCCGCTGCGTTTTCTTGCCTATGAACCACCCGCCAGCC |
| Internal bar code: | GCGGGGGGATAAGCAGGTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5015 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 125 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 125 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTTCGGTCTTGGTCCTC |
| Suggested primer 2: | TCTTGCGGAAGTACTACGGC |