| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.001692 |
| Chromosome: | chromosome 3 |
| Location: | 6868394 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197700 | HLM8 | (1 of 1) PTHR13793//PTHR13793:SF100 - PHD FINGER PROTEINS // HISTONE-LYSINE N-METHYLTRANSFERASE ATX1-RELATED; Histone-lysine N-methyltransferase | outside_mRNA |
| Cre03.g197750 | GPX3 | Glutathione peroxidase 3; (1 of 1) PTHR11592//PTHR11592:SF39 - GLUTATHIONE PEROXIDASE // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTAACAAGTGGTATACAAAAGGAAATGTAGTAAGGTATCAAGGGCGCC |
| Internal bar code: | TCGGTTTTAGCATTGGGAATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5769 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTGACGTACGACTACCG |
| Suggested primer 2: | AGAACCGCCTTGTCCTTGAG |