Insertion junction: CLIP2.001732_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.001732
Chromosome:chromosome 1
Location:4886352
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g034050 (1 of 1) PTHR11751:SF428 - PROTEIN NKAT-1-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACACAGGCGCCGTCTCGCAGCGGACGTGGTCACGTGTCTCATGAAGGTC
Internal bar code:TGTCAAGTTTAGGCTCTTTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2570
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:90
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:90

Suggested primers for confirmation by PCR

Suggested primer 1:TGAAAGCTTCGCCTGTTTGC
Suggested primer 2:AGGGAAGGAGATACGCAGGT