| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.001748 |
| Chromosome: | chromosome 1 |
| Location: | 5348747 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037700 | FAD3,FAD9,delta4FAD | (1 of 2) PTHR19353//PTHR19353:SF30 - FATTY ACID DESATURASE 2 // FATTY ACID DESATURASE 2; Delta4-fatty acid desaturase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCGCAATGGCAGGCGCTCAGGCCATCGGGTCATCCCTTGGGTCATCC |
| Internal bar code: | CAAGAGAGGGTTCTCAGATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3015 |
| LEAP-Seq percent confirming: | 82.0225 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGATGGCGAGTCTGCT |
| Suggested primer 2: | GGTATGTCCTCGAGAGCTGC |