Insertion junction: CLIP2.001789_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.001789
Chromosome:chromosome 7
Location:1097935
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g320350 CDJ5 (1 of 3) PF00226//PF13370 - DnaJ domain (DnaJ) // 4Fe-4S single cluster domain of Ferredoxin I (Fer4_13); Chloroplast DnaJ-like protein 5 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATATGGGGCGCCAGCGTCGGCGGGAAACAACATGATCTCGCACAGTGGCC
Internal bar code:GTTGCTCTGATTAGCTGGATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:726
LEAP-Seq percent confirming:57.1429
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TTAGGGGGTATGGGCGGTTA
Suggested primer 2:TAGCGCTGGTGTTGTGTGAT