Insertion junction: CLIP2.001810_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.001810
Chromosome:chromosome 17
Location:3540483
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g724700 PAO3,Tic55-like3,TIC55-3,PAO1 Pheophorbide a oxygenase-related protein; (1 of 3) K13071 - pheophorbide a oxygenase (PAO, ACD1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCCCAGCCCGCCGCGCTGCTGTCCGGCAGCACCCACAGCAGGCCGTGC
Internal bar code:TTGTCTGCACGGTCTAAACCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3755
LEAP-Seq percent confirming:95.9184
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:AGGACGGCAAGTACCAATGG
Suggested primer 2:CGTTGAGGCACAAGAGAGGT