| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.001819 |
| Chromosome: | chromosome 17 |
| Location: | 3540472 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724700 | PAO3,Tic55-like3,TIC55-3,PAO1 | Pheophorbide a oxygenase-related protein; (1 of 3) K13071 - pheophorbide a oxygenase (PAO, ACD1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCGTACCTTCTCCCAGCCCGCCGCGCTGCTGTCCGGCAGCACCCACA |
| Internal bar code: | CTTGTTGTTTTTTGAGGTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2986 |
| LEAP-Seq percent confirming: | 80.4878 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACGGCAAGTACCAATGG |
| Suggested primer 2: | CGTTGAGGCACAAGAGAGGT |