| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.001821 |
| Chromosome: | chromosome 12 |
| Location: | 4950581 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524500 | RMT2 | Putative protein N-methyltransferase; (1 of 2) PTHR13271//PTHR13271:SF30 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED | 5'UTR |
| Cre12.g524550 | (1 of 2) PTHR14614//PTHR14614:SF3 - HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGACGAGCCCACGGATCGATGGACCTTGCATGCAGCTGAGGAGTTGA |
| Internal bar code: | AAAAAATAGGTGTCATAGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2619 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCAGGTCAGATCTACCTC |
| Suggested primer 2: | GCAGCTCCTTCAGGCCTTTA |