Insertion junction: CLIP2.001825_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.001825
Chromosome:chromosome 2
Location:1660438
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g085450 CPX1 (1 of 2) 1.3.3.3 - Coproporphyrinogen oxidase / Coproporphyrinogenase; Coproporphyrinogen III oxidase 3'UTR
Cre02.g800180 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCTAGGTGTGTAACTCGGGGGTCCAGAGTGCAGTCAGTCCACACTGGA
Internal bar code:TAGATTATACTACACCTTCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1280
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:AACGTGGTGGAGGCATACTG
Suggested primer 2:CAACTTGCCTTGCCACTGTC