Insertion junction: CLIP2.001826_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.001826
Chromosome:chromosome 2
Location:6062936
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g119100 CER2 (1 of 2) K15889 - prenylcysteine alpha-carboxyl methylesterase (PCME); Carboxyesterase-related protein/arylformamidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCTTGGGCGATGATGGCAGAGGACACAGCAGCGCTGCCGCCGCCGCCG
Internal bar code:GGAGGCGCAAATGAGTCAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1913
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TCTACGTCACTGGTGTGTGC
Suggested primer 2:CGCATTCGAGAGTGCACATG