| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.001828 |
| Chromosome: | chromosome 16 |
| Location: | 2420884 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659850 | CGL37 | conserved protein related to shikimate kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACGGGGCAAGTCGCGCACATGGCGCGTACATGCCCGTAGCCCTGGCCC |
| Internal bar code: | AATCGCAGGAATACACGAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 76.9231 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTGACACCCACCAATCC |
| Suggested primer 2: | GAGTTCACACGTGAGCAGGA |