Insertion junction: CLIP2.001834_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.001834
Chromosome:chromosome 12
Location:5758913
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g532600 CGL44 RabGAP/TBC Domain Protein; (1 of 14) PF00566 - Rab-GTPase-TBC domain (RabGAP-TBC) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGACAACCCTACCAGGTCAGCACGCACGCGACGGGATTCCGCGCGCGAT
Internal bar code:CCACATATCTAATGGATTAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2326
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GGGAGATCACAACAGTCGCA
Suggested primer 2:AGGGCATCTCTCTGAGCTGA