Insertion junction: CLIP2.001834_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.001834
Chromosome:chromosome 12
Location:5758917
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g532600 CGL44 RabGAP/TBC Domain Protein; (1 of 14) PF00566 - Rab-GTPase-TBC domain (RabGAP-TBC) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGCACGTCGTGTCTTCCGGTCTACTGGAGCCCAACCCTGAAGGCCCG
Internal bar code:TCACATAGTCCCAGGGATCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1173
LEAP-Seq percent confirming:54.1667
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGCATCTCTCTGAGCTGA
Suggested primer 2:GGGAGATCACAACAGTCGCA