| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.001843 |
| Chromosome: | chromosome 6 |
| Location: | 1457999 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260250 | CEMA,CEM1,YCF10 | putative proton extrusion protein cemA, chloroplastic; (1 of 1) PTHR33650:SF1 - CEMA-LIKE PROTON EXTRUSION PROTEIN-LIKE PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGATCCAAGCCCTCGCGCACCGCATCCCAGTCCAGCAGCTGCAGGTC |
| Internal bar code: | TGGAAGTGTGGCTTACTCCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2322 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATTACGCCGGTGAGTGAA |
| Suggested primer 2: | CGAGTGGGGTTAGGGGAGTA |