Insertion junction: CLIP2.001849_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.001849
Chromosome:chromosome 10
Location:2149443
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g433350 (1 of 19) PTHR31251:SF1 - SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 10-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATCAATAGCGGCGCAGCTGGTTCCGTGTGCGCTGACATCCCCTCACCT
Internal bar code:GCATACAGATCGTGATGAATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:596
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGCTACTGCTGCTCCTAG
Suggested primer 2:AGACGCCAGTGTACAACAGG