Insertion junction: CLIP2.001853_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.001853
Chromosome:chromosome 13
Location:2424496
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g579450 CST1 Chlamydomonas-specific membrane transporter of unknown function intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAATTCTGTGGCCGCGTACGCGTGCTTTTACGTGTCACAAACATGGTTTG
Internal bar code:GACGACAGGCCATGTTATGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2056
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGAAGGAGTTGCGGAACT
Suggested primer 2:TAAACCGCAAAACCGCTTCG