Insertion junction: CLIP2.001856_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.001856
Chromosome:chromosome 12
Location:426086
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g495100 PSR1 (1 of 1) PF00249//PF14379 - Myb-like DNA-binding domain (Myb_DNA-binding) // MYB-CC type transfactor, LHEQLE motif (Myb_CC_LHEQLE); Phosphorus starvation response 1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTGGATCGAAAGCTTGCTGCGGCCCCGCAGACGCATGCCAGTCGTCAA
Internal bar code:ATCGTCCCCTAAGACGAGAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:864
LEAP-Seq percent confirming:40.9091
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GGTACGGATTGCAACATCGC
Suggested primer 2:GAGACCATATCCACCCAGCG