| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.001862 |
| Chromosome: | chromosome 12 |
| Location: | 3144253 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500650 | RNB2 | (1 of 1) PTHR23355//PTHR23355:SF39 - RIBONUCLEASE // SUBFAMILY NOT NAMED; chloroplast RNase II | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGCGCGAGGAGATGGGGCTGGTGGCGTTCCTGCTTGAGGACCAGGG |
| Internal bar code: | GCCCAAGAGGGATCGCCCGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 56 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTTGACCACAACCTGCG |
| Suggested primer 2: | ATCCCTTTGCGACTGCTCAA |