Insertion junction: CLIP2.001869_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.001869
Chromosome:chromosome 4
Location:1685571
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g212401 UBC15 (1 of 2) K10586 - baculoviral IAP repeat-containing protein 6 (apollon) (BIRC6, BRUCE); E2 Ubiquitin conjugating enzyme intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACCACCCCGGTACGCCCCGCACCTGCCAACGCATTCTTCCCTGCCACT
Internal bar code:AATCGTGTTTGCGGAACAGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:238
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CATGATCCTAGTGGCCGACC
Suggested primer 2:CAGCCAATAGCCTTCCCCAA