| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.001870 |
| Chromosome: | chromosome 4 |
| Location: | 3218968 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226450 | PRP17 | (1 of 1) K12816 - pre-mRNA-processing factor 17 (CDC40, PRP17); Nuclear pre-mRNA splicing factor, component of the spliceosome | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCGGGCACAGAACCCCCCCCCCCGCCCCGCCTCCCGCTCACCTATCC |
| Internal bar code: | CGGGTCGGTAGCGTATGTCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 232 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGTGTGGGTGGTATTGG |
| Suggested primer 2: | GGTTGGGTGTTGTTTCGAGC |