| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.001875 |
| Chromosome: | chromosome 1 |
| Location: | 3100667 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019700 | PAP7 | Class-II RNA nucleotidyl transferase 7; (1 of 12) 2.7.7.19 - Polynucleotide adenylyltransferase / RNA adenylating enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCTAGCGCCCCCACGCCAGGGCCCTCAAGAAGCCTATCAGTATTTGC |
| Internal bar code: | TCCACAGCGGTGACGCGATGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2173 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCTTACGCACGTACACA |
| Suggested primer 2: | AGGGCGCGTACCAAGTAAAA |