Insertion junction: CLIP2.001876_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.001876
Chromosome:chromosome 13
Location:2172263
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g577850 FKB20,FKB20B,FKB20-2 (1 of 1) PTHR10516:SF178 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP20-2, CHLOROPLASTIC; peptidyl-prolyl cis-trans isomerase, FKBP-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAACGCAATGTACGTTTTGATTTTGCCTCCTGCACAGGTCGCCCCGCC
Internal bar code:AGAAAGGACACTGTTTAACAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3629
LEAP-Seq percent confirming:98.8372
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:86

Suggested primers for confirmation by PCR

Suggested primer 1:TGATACATCTCGTGACGCCG
Suggested primer 2:GAGCATTGTATGGCAGCGTG