Insertion junction: CLIP2.001883_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.001883
Chromosome:chromosome 8
Location:2489551
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g372450 PSBQ1,OEE3, PSBQ (1 of 1) K08901 - photosystem II oxygen-evolving enhancer protein 3 (psbQ); Photosystem II Oxygen Evolution Enhancer protein 3 outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTCACACGTGTTCTAGGTTCCGACGCGTCACACCACCGCTTGCATTGC
Internal bar code:TAGCGTCCTGTATCCTATGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:138
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:AAAAGGTGCGGCATGTTTCC
Suggested primer 2:CGGTGTACCTCCTATTCCGC