Insertion junction: CLIP2.001912_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.001912
Chromosome:chromosome 16
Location:4760444
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g687900 LHCA7 Light-harvesting protein of photosystem I; (1 of 3) K08911 - light-harvesting complex I chlorophyll a/b binding protein 5 (LHCA5) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCGTACCGAGATGGCGAGAGATCTGCCGACCGGAGAGGCACATTGGCT
Internal bar code:AAGATAATGGTACTGCGTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3054
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCTTGAAAGATAGCGCC
Suggested primer 2:ACTGGTGTGGATCTTCTGCG