Insertion junction: CLIP2.001912_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.001912
Chromosome:chromosome 16
Location:4760462
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g687900 LHCA7 Light-harvesting protein of photosystem I; (1 of 3) K08911 - light-harvesting complex I chlorophyll a/b binding protein 5 (LHCA5) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCAGCAATTTTCGTTTGTCCATACAAGGTGCCAGTATGATCAGGTATT
Internal bar code:AAGATAATGGTACTGCGTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:93
LEAP-Seq percent confirming:16.6667
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGGTGTGGATCTTCTGCG
Suggested primer 2:GCTGCTTGAAAGATAGCGCC