| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.001913 |
| Chromosome: | chromosome 17 |
| Location: | 3402460 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723250 | CPP1,CPE1 | chloroplast processing enzyme; (1 of 1) PTHR11851//PTHR11851:SF152 - METALLOPROTEASE // PROTEIN UCR-2.3 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAATACAAAAAGCTGTACCGGTGTGCGAGCGCAGTGATACACGTCCTG |
| Internal bar code: | CTCAACCCGGTGTGCCGCTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2950 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGACGAGGACGCCATTTT |
| Suggested primer 2: | CAACCCCTCTCACATGCCAT |