| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.001926 |
| Chromosome: | chromosome 5 |
| Location: | 3582889 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234100 | CYP745A1,CYP17 | Cytochrome P450, CYP197 superfamily; (1 of 1) PTHR24305:SF59 - CYTOCHROME P450 313A1-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTTCGGATGATGGAAGGCTTGGGAGGGGCGACGTGACAGCGGGCATG |
| Internal bar code: | GACTTTTGATCATATGCAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 604 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATTTGCTTGGTCCGTGC |
| Suggested primer 2: | CCAACACCTCCCACATCTCC |