Insertion junction: CLIP2.001928_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.001928
Chromosome:chromosome 11
Location:4260672
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g482001 FAP208 Ankyrin Repeat Flagellar Associated Protein 208; (1 of 1) K13118 - protein DGCR14 (DGCR14) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCACTCAGTTACCCGCATCCAACACCAATCGAAACACTGCTACAACAC
Internal bar code:AAATATAGTTTCTCTTTGCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:198
LEAP-Seq percent confirming:76.4706
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CACACTCGCCATTCCTCTGA
Suggested primer 2:GCTTTGCTTCATAGGAGCGC