Insertion junction: CLIP2.001929_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.001929
Chromosome:chromosome 16
Location:8015755
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g692228 MARS1 Mutant Affecting Retrograde Signaling 1; (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATACAATGGAGACGCGTACTTATGTGCGCGGACGGGCCAAAGCCATCAA
Internal bar code:GTCCGCGATCTCGATTTATCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2639
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGTGTCAACTCCTCGCTC
Suggested primer 2:AGGTGAAGTCTGAAGGCACG