Insertion junction: CLIP2.001931_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.001931
Chromosome:chromosome 3
Location:3810630
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g169900 (1 of 4) IPR023222 - PsbQ-like domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCACCTTCTCCACCTTGTCCTCGGGAACACCCTGCGGCGTTCCGCACGC
Internal bar code:CACCATACCGGTCATAGGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2964
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:AACCTGCCTCACAAACCACA
Suggested primer 2:CCAACCCAGATGATTGCCCT