Insertion junction: CLIP2.001947_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.001947
Chromosome:chromosome 1
Location:1747074
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g009650 BUG25 (1 of 11) IPR001471//IPR016177//IPR031112 - AP2/ERF domain // DNA-binding domain // AP2-like ethylene-responsive transcription factor; basal body protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGCGCCTCGCCTTCTCGCGGGCTATGCCACCTTGTTCATTACGATGCA
Internal bar code:GGACACCTAAAGGTCGTCCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1176
LEAP-Seq percent confirming:95.4545
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGGATGGACGCTGTTTGA
Suggested primer 2:TCACACAGCATCGTCGTGAA