Insertion junction: CLIP2.001986_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.001986
Chromosome:chromosome 2
Location:6836109
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388356 OPR7,TBC2 Translation factor for chloroplast psbC RNA; (1 of 19) PTHR21228//PTHR21228:SF20 - FAST LEU-RICH DOMAIN-CONTAINING // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCACGCTAGCCCGAACGCCCCGGCCAGTGGCTTGCACCCGACCGCTAGC
Internal bar code:CCCGCGGAGGTGTACCTAGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:4077
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:58
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:TCACGAAGCTGATTGCCAGT
Suggested primer 2:GAGGATGCGTTCGTGATTGC