Insertion junction: CLIP2.001996_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.001996
Chromosome:chromosome 1
Location:7556994
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g053000 GPD1,GPDH2,GPD2 (1 of 3) K00006 - glycerol-3-phosphate dehydrogenase (NAD+) (GPD1); Glycerol-3-phosphate dehydrogenase/dihydroxyacetone-3-phosphate reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACAACAGGACTCCACACCCTACCCTGTTCCACTTTCTCCCCTCCCCT
Internal bar code:GGATCCCGTTATTTTTCACTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3544
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTGTGTAGGTGTGCTCT
Suggested primer 2:CGGTCGTGGCTGCATACTAT