Insertion junction: CLIP2.002001_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.002001
Chromosome:chromosome 4
Location:667239
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre04.g217949 AXL6 (1 of 1) 2.4.1.37 - Fucosylgalactoside 3-alpha-galactosyltransferase / galactosyltransferase; Arabinose chain extension enzyme like protein 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGCAGGTTCTGGGCAGAGTTGGTTAAGCGGAGCCACGGACGGATGCA
Internal bar code:CAGTACTGGTGTAGTGTAGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:180
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCCCTTATCCACCCCTA
Suggested primer 2:AAACATCCTTGCAACCACGC