| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.002005 |
| Chromosome: | chromosome 13 |
| Location: | 1550999 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572900 | THO3 | (1 of 1) 3.4.24.70 - Oligopeptidase A; Oligoendopeptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCCGGCTGCGTCAGCAGCTTCTTGTAGGCCTGGAGGGGTGGTGGTGG |
| Internal bar code: | GCGGAACAGTATGTGCAAATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGGCGATCAAGTTGGTC |
| Suggested primer 2: | AAGAGCTGGAGAGAGGGAGG |