Insertion junction: CLIP2.002009_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.002009
Chromosome:chromosome 16
Location:4814474
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g687500 ARP2 Actin-related protein; (1 of 1) K17260 - actin-related protein 2 (ACTR2, ARP2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAACAGATTGCGAGCAGGGTCGTGGGTCTTCGGTGGGGATTGTTGTCG
Internal bar code:CTAGCAGGGAAGCGACGAAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:1491
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GCACACACACATCCACACAC
Suggested primer 2:TTAACCCAATCCGCATGGCT