Insertion junction: CLIP2.002031_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.002031
Chromosome:chromosome 16
Location:1939072
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g656200 (1 of 1) PF00612//PF13424 - IQ calmodulin-binding motif (IQ) // Tetratricopeptide repeat (TPR_12) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGCTTCTGCAGCACTTCATAGGTGCGCCCTGGCATTGGCTGGGATTGG
Internal bar code:CTAGTGAACGTGTGCGTATACA

Confirmation - LEAP-Seq

LEAP-Seq distance:3416
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGCGGCTCATACACAATG
Suggested primer 2:TGGAAATGGCGAGCTCTGAG