Insertion junction: CLIP2.002038_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.002038
Chromosome:chromosome 2
Location:1312096
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g082550 ZEP,ZEP1 Zeaxanthin epoxidase 1; (1 of 1) K09838 - zeaxanthin epoxidase (ZEP, ABA1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAACCACCTCGCTCAAACTCCAACCGGGACAGGACATGCACAAACGAC
Internal bar code:CGGTCTCATTCATTGGAACAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3488
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGAGGAATGGGAGGAGAG
Suggested primer 2:TTGCTAGGACTCACTGTGCG