| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.002041 |
| Chromosome: | chromosome 5 |
| Location: | 2176720 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237350 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGTAGCAAGAAGGGACAAGGCATTGGTGCGTCGTTCGCGCGCTGTTA |
| Internal bar code: | ACGCCTGGCCCTAAGTGATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2181 |
| LEAP-Seq percent confirming: | 97.3684 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTCGGTCGGGACTTACCA |
| Suggested primer 2: | CCTTCTGAGCAGGAACCAGG |