| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.002062 |
| Chromosome: | chromosome 6 |
| Location: | 2020074 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265550 | CDPK12 | (1 of 1) PF07714//PF13202 - Protein tyrosine kinase (Pkinase_Tyr) // EF hand (EF-hand_5); Calcium/calmodulin-dependent protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTGGTAGCAAGGCTTCCGCGCCGCACGCACGTCACAACGCACGAATG |
| Internal bar code: | AGCCGAGCTGCGTTTGGGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1132 |
| LEAP-Seq percent confirming: | 34.375 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGCAACTTGCTCGTGCT |
| Suggested primer 2: | TCAACGCAACGATGTCTCCA |